PA5-53954

antibody from Invitrogen Antibodies

Targeting: AQP1 CHIP28, CO

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-53954

Provider

Invitrogen Antibodies

Product name

Aquaporin 1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: PRSSDLTDRV KVWTSGQVEE YDLDADDINS RVEMKPK Highest antigen sequence identity to the following orthologs: Mouse - 95%, Rat - 95%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.08 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Hamster organotypic kidney culture model of early-stage SARS-CoV-2 infection highlights a two-step renal susceptibility.

Shyfrin SR, Ferren M, Perrin-Cocon L, Espi M, Charmetant X, Brailly M, Decimo D, Iampietro M, Canus L, Horvat B, Lotteau V, Vidalain PO, Thaunat O, Mathieu C
Journal of tissue engineering 2022 Jan-Dec;13:20417314221122130

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Aquaporin 1 in control (vector only transfected HEK293T lysate) and aQP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a Aquaporin 1 Polyclonal Antibody (Product # PA5-53954).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Aquaporin 1 Polyclonal Antibody (Product # PA5-53954) and a 34 kDa band corresponding to Aquaporin 1 was observed across cell lines tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), MCF7 (Lane 2), A549 (Lane 3), ACHN (Lane 4), and MDA-MB-231 (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.2 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of Aquaporin 1 in human cell line U-2 OS shows positivity in plasma membrane. Samples were probed using an Aquaporin 1 Polyclonal Antibody (Product # PA5-53954).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Aquaporin 1 in human kidney and liver tissues using Aquaporin 1 Polyclonal Antibody (Product # PA5-53954). Corresponding AQP1 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Aquaporin 1 in human rectum using Aquaporin 1 Polyclonal Antibody (Product # PA5-53954) shows moderate positivity in apical membranes in glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Aquaporin 1 in human lung using Aquaporin 1 Polyclonal Antibody (Product # PA5-53954) shows strong membranous positivity in endothelial cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Aquaporin 1 in human kidney using Aquaporin 1 Polyclonal Antibody (Product # PA5-53954) shows moderate to strong positivity in apical membranes in cells in tubules.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Aquaporin 1 in human liver using Aquaporin 1 Polyclonal Antibody (Product # PA5-53954) shows no positivity in hepatocytes as expected.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4. Tropism and dissemination of SARS-CoV-2 in hamster organotypic kidney cultures. Organotypic kidney cultures (OKC) were infected with 1000 pfu of wild-type SARS-CoV-2 and fixed in 4% paraformaldehyde at 1 or 4 days post infection (dpi). (a and b) OKC sections stained against SARS-CoV-2 nucleoprotein (NP) and CD34 (marker of endothelial cells) at day 1 and 4 post infection ((a and b), respectively). (a) is showing the edge of the slice. (b) is showing both the edge and the center of the slice, demonstrating the spread of infection toward the center. (c) OKC sections stained against SARS-CoV-2 NP and (c) aquaporin-1 (marker of proximal tubular epithelial cells). Immunofluorescence images were acquired using confocal microscopy and is representative of three independent experiments. Colocalization of cell type markers (red) with SARS-CoV-2 NP (green) is denoted with arrows. Scalebar = 100 um.