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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- TA502357 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502357, RRID:AB_11124856
- Product name
- AQP1 (Aquaporin 1) mouse monoclonal antibody, clone OTI2D10 (formerly 2D10)
- Antibody type
- Monoclonal
- Description
- AQP1 (Aquaporin 1) mouse monoclonal antibody, clone OTI2D10 (formerly 2D10)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- AQP1
- Isotype
- IgG
- Antibody clone number
- OTI2D10
- Vial size
- 100 µl
- Concentration
- 0.47 mg/ml
Submitted references Specific aquaporins facilitate Nox-produced hydrogen peroxide transport through plasma membrane in leukaemia cells.
Vieceli Dalla Sega F, Zambonin L, Fiorentini D, Rizzo B, Caliceti C, Landi L, Hrelia S, Prata C
Biochimica et biophysica acta 2014 Apr;1843(4):806-14
Biochimica et biophysica acta 2014 Apr;1843(4):806-14
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AQP1 (RC205304, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AQP1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-AQP1 monoclonal antibody at 1:200 dilution.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Western Blot of AQP1 protein level by using anti-AQP1 antibody in human B1647 cells.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-AQP1 mouse monoclonal antibody (TA502357) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AQP1(RC205304).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-AQP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502357)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-AQP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502357)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-AQP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502357)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-AQP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502357)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC205304 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AQP1 antibody(TA502357), and then analyzed by flow cytometry.
- Validation comment
- FC
