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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [7]
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- Validations
- Immunohistochemistry [3]
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- Product number
- NB600-749 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-749, RRID:AB_10128910
- Product name
- Mouse Monoclonal Aquaporin 1/AQP1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Antibody recognises an epitope within the cytoplasmic domain of the water-specific channel aquaporin 1.
- Reactivity
- Human, Mouse, Rat, Rabbit, Zebrafish
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.05 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Generation and Characterisation of a Pax8-CreERT2 Transgenic Line and a Slc22a6-CreERT2 Knock-In Line for Inducible and Specific Genetic Manipulation of Renal Tubular Epithelial Cells.
A minimally invasive, lentiviral based method for the rapid and sustained genetic manipulation of renal tubules.
Signaling from the secretory granule to the nucleus: Uhmk1 and PAM.
Expression of aquaporin isoforms during human and mouse tooth development.
Development and characterisation of a monoclonal antibody family against aquaporin 1 (AQP1) and aquaporin 4 (AQP4).
Active water selective channels in the stomach: investigation of aquaporins after ethanol and capsaicin treatment in rats.
Active water selective channels in the stomach: investigation of aquaporins after ethanol and capsaicin treatment in rats.
Espana-Agusti J, Zou X, Wong K, Fu B, Yang F, Tuveson DA, Adams DJ, Matakidou A
PloS one 2016;11(2):e0148055
PloS one 2016;11(2):e0148055
A minimally invasive, lentiviral based method for the rapid and sustained genetic manipulation of renal tubules.
Espana-Agusti J, Tuveson DA, Adams DJ, Matakidou A
Scientific reports 2015 Jun 5;5:11061
Scientific reports 2015 Jun 5;5:11061
Signaling from the secretory granule to the nucleus: Uhmk1 and PAM.
Francone VP, Ifrim MF, Rajagopal C, Leddy CJ, Wang Y, Carson JH, Mains RE, Eipper BA
Molecular endocrinology (Baltimore, Md.) 2010 Aug;24(8):1543-58
Molecular endocrinology (Baltimore, Md.) 2010 Aug;24(8):1543-58
Expression of aquaporin isoforms during human and mouse tooth development.
Felszeghy S, Módis L, Németh P, Nagy G, Zelles T, Agre P, Laurikkala J, Fejerskov O, Thesleff I, Nielsen S
Archives of oral biology 2004 Apr;49(4):247-57
Archives of oral biology 2004 Apr;49(4):247-57
Development and characterisation of a monoclonal antibody family against aquaporin 1 (AQP1) and aquaporin 4 (AQP4).
Nagy G, Szekeres G, Kvell K, Berki T, Németh P
Pathology oncology research : POR 2002;8(2):115-24
Pathology oncology research : POR 2002;8(2):115-24
Active water selective channels in the stomach: investigation of aquaporins after ethanol and capsaicin treatment in rats.
Bódis B, Nagy G, Németh P, Mózsik G
Journal of physiology, Paris 2001 Jan-Dec;95(1-6):271-5
Journal of physiology, Paris 2001 Jan-Dec;95(1-6):271-5
Active water selective channels in the stomach: investigation of aquaporins after ethanol and capsaicin treatment in rats.
Bódis B, Nagy G, Németh P, Mózsik G
Journal of physiology, Paris 2001 Jan-Dec;95(1-6):271-5
Journal of physiology, Paris 2001 Jan-Dec;95(1-6):271-5
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Slc22a6-CreERT2 mediated beta-galactosidase expression. beta-galactosidase positivity was exclusively found in the tubular epithelium of the kidney of tamoxifen treated mice (a); kidneys from vehicle treated mice were negative (b). Co-localisation studies revealed specific X-gal expression within proximal tubular epithelium (AQP1) with no expression detected in distal tubules (THP) or collecting ducts (AQP2). Scale bars, 100um. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0148055), licensed under a CC-BY licence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Intraparenchymal delivery of lentiviral vectors preferentially infects epithelial cells of both kidneys and urinary bladder. Confocal images of Strawberry expression in renal sections of ELS infected kidneys 60 days post injection. Sections were stained with the indicated antibodies and merged images with DAPI (blue) are presented. Preferential transduction was observed in proximal (AQP1) and distal (THP) renal tubular epithelial cells and renal fibroblasts (CD73) (white arrowheads). Strawberry was not localized to collecting ducts (AQP2) or podocytes (Nephrin) (white arrows). Scale bars, 50 um. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/doifinder/10.1038/srep11061), licensed under a CC-BY licence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Pax8-CreERT2 mediated beta-galactosidase expression. beta-galactosidase positivity was exclusively found in the tubular epithelium of the kidney of tamoxifen treated mice, with no staining observed in glomeruli or bloods vessels (a, framed area is shown enlarged in b). Kidneys from vehicle treated mice were negative (c). Co-localisation studies revealed X-gal expression within tubules of all renal tubular compartment (proximal tubules-AQP1, distal tubules-THP and collecting ducts-AQP2). Scale bars, 100um. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0148055), licensed under a CC-BY licence.
