Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-71646 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-PCDHAC2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.56 mg/ml
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PCDHAC2 in NCI-H460 cells using confocal microscopy. Samples were incubated with PCDHAC2 H1 polyclonal antibody (Product # PA5-71646) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) as a secondary antibody. Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PCDHAC2 in paraffin-embedded human brain tissue. Samples were incubated with PCDHAC2 polyclonal antibody (Product # PA5-71646) followed by a peroxidase-conjugate secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of PCDHAC2 in NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). Samples were incubated with PCDHAC2 polyclonal antibody (Product # PA5-71646) followed by a FITC conjugated donkey anti-rabbit secondary antibody.