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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AP53204PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP53204PU-N, RRID:AB_11145206
- Product name
- anti PCDHAC2 (Center)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide - KLH conjugated - corresponding to the central region (between 623-652aa) of human PCDHAC2.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis in NCI-H460 cell line lysates (35ug/lane) using PCDHAC2 antibody Cat.-No.AP53204PU-N. This demonstrates the PCDHAC2 antibody detected the PCDHAC2 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of NCI-H460 cells using PCDHAC2 antibody Cat.-No.AP53204PU-N, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in human brain tissue (formalin-fixed, paraffin-embedded) using PCDHAC2 antibody Cat.-No.AP53204PU-N, followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of PCDHAC2 antibody for IHC. Clinical relevance has not been evaluated.
