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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00054474-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00054474-M01, RRID:AB_425989
- Product name
- KRT20 monoclonal antibody (M01), clone 2G3-1C8
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant KRT20.
- Antigen sequence
MDFSRRSFHRSLSSSLQAPVVSTVGMQRLGTTPSV
YGGAGGRGIRISNSRHTVNYGSDLTGGGDLFVGNE
KMAMQNLNDRLASYLEKVRTLEQSNSKLEVQIKQW
YETNAPRAGRDYSAYYRQIEELRSQIKDAQLQNAR
CVLQIDNAKLAAEDFRLKYETERGIRLTVEADLQG
LNKVFDDLTLHKTDLEIQIEELNKDLALLKKEHQE
EVDGLHKHLGNTVNVEVDAAPGLNLGVIMNEMRQK
YEVMAQKNLQEAKEQFERQTAVLQQQVTVNTEELK
GTEVQLTELRRTSQSLEIELQSHLSMKESLEHTLE
ETKARYSSQLANLQSLLSSLEAQLMQIRSNMERQN
NEYHILLDIKTRLEQEIATYRRLLEGEDVKTTEYQ
LSTLEERDIKKTRKIKTVVQEVVDGKVVSSEVKEV
EENI- Isotype
- IgG
- Antibody clone number
- 2G3-1C8
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- KRT20 monoclonal antibody (M01), clone 2G3-1C8 Western Blot analysis of KRT20 expression in A-431 ( Cat # L015V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of KRT20 expression in transfected 293T cell line by KRT20 monoclonal antibody (M01), clone 2G3-1C8.Lane 1: KRT20 transfected lysate(48.5 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged KRT20 is approximately 0.03ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to KRT20 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to KRT20 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
