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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- UM800065 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#UM800065, RRID:AB_2629175
- Product name
- KRT20/CK20 mouse monoclonal antibody,clone UMAB172
- Antibody type
- Monoclonal
- Description
- KRT20/CK20 mouse monoclonal antibody,clone UMAB172
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- KRT20
- Isotype
- IgG
- Antibody clone number
- UMAB172
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
Submitted references Immunohistochemical Evaluation of Peri-Implant Soft Tissues around Machined and Direct Metal Laser Sintered (DMLS) Healing Abutments in Humans.
Mangano C, Mangano FG, Shibli JA, Roth LA, d' Addazio G, Piattelli A, Iezzi G
International journal of environmental research and public health 2018 Jul 30;15(8)
International journal of environmental research and public health 2018 Jul 30;15(8)
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Diluation: 1:500.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-KRT20 mouse monoclonal antibody. (UM800065; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-KRT20 mouse monoclonal antibody (UM800065). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
- Validation comment
- 10K-CHIP
