Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- M02828 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Cytokeratin 20 KRT20 Rabbit Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Monoclonal antibody for CYTOKERATIN 20/KRT20 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IP, IF, IHC, ICC, WB. Reactive species: Human, Rat CYTOKERATIN 20/KRT20 information: Molecular Weight: 48487 MW; Subcellular Localization: Cytoplasm ; Tissue Specificity: Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Antibody clone number
- CDI-11
- Vial size
- 100ug/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. Avoid repeated freezing and thawing.
Submitted references Pulmonary neuroendocrine cells sense succinate to stimulate myoepithelial cell contraction.
Yu W, Moninger TO, Rector MV, Stoltz DA, Welsh MJ
Developmental cell 2022 Sep 26;57(18):2221-2236.e5
Developmental cell 2022 Sep 26;57(18):2221-2236.e5
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon, using Cytokeratin 20 Antibody(M02828)KRT20 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-KRT20 Antibody (M02828)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image