Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- A02828-2 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Cytokeratin 20/Krt20 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for 20/Krt20 detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Identification of relevant prognostic values of cytokeratin 20 and cytokeratin 7 expressions in lung cancer.
Luo HT, Liang CX, Luo RC, Gu WG
Bioscience reports 2017 Dec 22;37(6)
Bioscience reports 2017 Dec 22;37(6)
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of CACO-2 cells using anti-Krt20 antibody (A02828-2). Overlay histogram showing CACO-2 cells stained with A02828-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Krt20 Antibody (A02828-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
- Additional image