LS-C815517

antibody from LSBio

Targeting: APOBEC3B FLJ21201, PHRBNL

Western blot (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C815517

Provider

LSBio

Product name

APOBEC3B Antibody (Preservative Free) LS-C815517

Antibody type

Polyclonal

Description

Protein A affinity chromatography

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

APOBEC3B was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. 4 uL anti-APOBEC3B rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-APOBEC3B rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 46 kDa. Observed band size: 46 kDa.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

APOBEC3B was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. 4 uL anti-APOBEC3B rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-APOBEC3B rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 46 kDa. Observed band size: 46 kDa.