LS-C815472

antibody from LSBio

Targeting: DES CMD1I, CSM1, CSM2

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Enhanced validation)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C815472

Provider

LSBio

Product name

DES / Desmin Antibody (Preservative Free) LS-C815472

Antibody type

Polyclonal

Description

Protein A affinity chromatography

Reactivity

Mouse

Host

Rabbit

Isotype

IgG

Storage

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

Immunoprecipitation

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

DES was immunoprecipitated using: Lane A: 0.5 mg Mouse heart tissue ysate Whole Cell Lysate. Lane B: 0.5 mg A431 Whole Cell Lysate. 4 uL anti-DES rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-DES rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 55 kDa.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

DES was immunoprecipitated using: Lane A: 0.5 mg Mouse heart tissue ysate Whole Cell Lysate. Lane B: 0.5 mg A431 Whole Cell Lysate. 4 uL anti-DES rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-DES rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 55 kDa.