TA328678

antibody from OriGene

Targeting: P2RY11 P2Y11

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA328678

Provider

OriGene

Product name

Rabbit Polyclonal Anti-P2Y11 Receptor

Antibody type

Polyclonal

Description

Rabbit Polyclonal Anti-P2Y11 Receptor

Host

Rabbit

Conjugate

Unconjugated

Epitope

P2RY11

Antibody clone number

NULL

Vial size

200 µl

Concentration

NULL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis human platelet lysates: 1. Anti-P2Y11 Receptor antibody, (1:200). 2. Anti-P2Y11 Receptor antibody, preincubated with the control peptide antigen.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Expression of P2Y11 receptor in human P2Y11-MDCK transfected cells. Immunocytochemical staining of paraformaldehyde-fixed and permeabilized human P2Y11-MDCK transfected cells. A. Staining using Anti-P2Y11 Receptor antibody, (1:200), followed by Alexa-488-conjugated goat-anti-rabbit secondary antibody. B. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merged images of panels A and B.

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

IHC staining of rat spleen using Anti-P2Y11 Receptor antibody. Secondary (activated) follicle in the spleen white pulp shows intense staining of Marginal Zone T lymphocytes (red arrow); note that cells in the red pulp (green arrow) and B lymphocytes in the germinal center (blue arrow) are not stained. Yellow arrows show mononuclear cells with intense stain, probably non specific due to very high intrinsic peroxidase activity. DAB product is brown and cresyl violet is used as the counterstain.

Validation comment

IHC