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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB3291 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3291, RRID:AB_1578077
- Product name
- NME2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of NME2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Nm23/NDP kinases in human male germ cells: role in spermiogenesis and sperm motility?
Expression of cell surface NM23 proteins of human leukemia cell lines of various cellular lineage and differentiation stages.
Human brain nucleoside diphosphate kinase activity is decreased in Alzheimer's disease and Down syndrome.
Munier A, Serres C, Kann ML, Boissan M, Lesaffre C, Capeau J, Fouquet JP, Lacombe ML
Experimental cell research 2003 Oct 1;289(2):295-306
Experimental cell research 2003 Oct 1;289(2):295-306
Expression of cell surface NM23 proteins of human leukemia cell lines of various cellular lineage and differentiation stages.
Okabe-Kado J, Kasukabe T, Honma Y
Leukemia research 2002 Jun;26(6):569-76
Leukemia research 2002 Jun;26(6):569-76
Human brain nucleoside diphosphate kinase activity is decreased in Alzheimer's disease and Down syndrome.
Kim SH, Fountoulakis M, Cairns NJ, Lubec G
Biochemical and biophysical research communications 2002 Aug 30;296(4):970-5
Biochemical and biophysical research communications 2002 Aug 30;296(4):970-5
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of NME2 polyclonal antibody (Cat # PAB3291) in mouse brain tissue lysate. NME2 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of NME2 polyclonal antibody (Cat # PAB3291) in NCI-H460 cell line lysates (35 ug/lane). NME2 (arrow) was detected using the purified polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with NME2 polyclonal antibody (Cat # PAB3291) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
