Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AF6665 - Provider product page
- Provider
- R&D Systems
- Product name
- Human NM23-H2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human NM23-H2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human NM23-H1 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P22392
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human NM23-H2 by Western Blot. Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line and MCF-7 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human NM23-H2 Polyclonal Antibody (Catalog # AF6665) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for NM23-H2 at approximately 20 and 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- NM23-H2 in MCF-7 Human Cell Line. NM23-H2 was detected in immersion fixed MCF-7 human breast cancer cell line using Sheep Anti-Human NM23-H2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6665) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.