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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- AVARP00009_P050 - Provider product page
- Provider
- Aviva Systems Biology
- Proper citation
- Aviva Systems Biology Cat#AVARP00009_P050, RRID:AB_2047253
- Product name
- NME1 antibody - N-terminal region (AVARP00009_P050)
- Antibody type
- Polyclonal
- Antigen
- The immunogen is a synthetic peptide directed towards the N terminal region of human NME1
- Description
- Affinity Purified
- Reactivity
- Human, Mouse, Rat, Bovine, Guinea Pig, Horse, Rabbit
- Host
- Rabbit
- Vial size
- 100 μl
- Concentration
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- Storage
- For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis using Anti-NME1 antibody detects protein at predicted molecular weight using Fetal Heart Tissue as a positive control. Tissue/Lysate Concentration: 1ug/ml Antibody Concentration: 1.0ug/ml
- Sample type
- Fetal Heart Tissue
- Validation comment
- Western blot analysis detects protein at predicted molecular weight
- Primary Ab dilution
- 1.0ug/ml
- Other comments
- Lysate concentration 1ug/ml
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Immunohistochemistry staining of Paraffin Embedded Pancreas Tissue using Anti-NME1 antibody at a concentration of 4-8µg/ml
- Sample type
- Pancreas Tissue
- Validation comment
- The subcellular location is supported by literature.
- Primary Ab dilution
- 4-8µg/ml
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Immunohistochemistry staining of Paraffin Embedded Small Intestine Tissue using Anti-NME1 antibody at a concentration of 4-8µg/ml
- Sample type
- Small Intestine Tissue
- Validation comment
- The subcellular location is supported by literature.
- Primary Ab dilution
- 4-8µg/ml
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Immunohistochemistry staining of Paraffin Embedded Small Intestine Tissue using Anti-NME1 antibody at a concentration of 4-8µg/ml
- Sample type
- Small Intestine Tissue
- Validation comment
- The subcellular location is supported by literature.
- Primary Ab dilution
- 4-8µg/ml
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- DB Suggested Anti-Nme1 antibody Titration: 0.5 µg/mlPositive Control: Recomninant human NME2 protein
