11086-2-AP

antibody from Invitrogen Antibodies

Targeting: NME1 NDPKA, NM23, NM23-H1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11086-2-AP

Provider

Invitrogen Antibodies

Product name

NME1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MANCERTFI AIKPDGVQRG LVGEIIKRFE QKGFRLVGLK FMQASEDLLK EHYVDLKDRP FFAGLVKYMH SGPVVAMVWE GLNVVKTGRV MLGETNPADS KPGTIRGDFC IQVGRNIIHG SDSVESAEKE IGLWFHPEEL VDYTSCAQNW IYE (1-152 aa encoded by BC018994)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.35 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of 10086-2-AP (NME1 antibody).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF7 cells were subjected to SDS PAGE followed by western blot with 11086-2-AP (NME1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 11086-2-AP (NME1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 11086-2-AP (NME1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells using 11086-2-AP (NME1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas cancer using 11086-2-AP (NME1 antibody) at dilution of 1:100 (under 25x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer using 11086-2-AP (NME1 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer using 11086-2-AP (NME1 antibody) at dilution of 1:100 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-NME1 (IP:11086-2-AP, 4ug; Detection:11086-2-AP 1:500) with A549 cells lysate 2800ug.