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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB3851 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3851, RRID:AB_1575168
- Product name
- GFAP polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of GFAP.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Molecular genetic study in Japanese patients with Alexander disease: a novel mutation, R79L.
Detection of glial fibrillary acidic protein and neurofilaments in the cerebrospinal fluid of patients with neurocysticercosis.
A new splice variant of glial fibrillary acidic protein, GFAP epsilon, interacts with the presenilin proteins.
Shiroma N, Kanazawa N, Kato Z, Shimozawa N, Imamura A, Ito M, Ohtani K, Oka A, Wakabayashi K, Iai M, Sugai K, Sasaki M, Kaga M, Ohta T, Tsujino S
Brain & development 2003 Mar;25(2):116-21
Brain & development 2003 Mar;25(2):116-21
Detection of glial fibrillary acidic protein and neurofilaments in the cerebrospinal fluid of patients with neurocysticercosis.
Quintanar JL, Franco LM, Salinas E
Parasitology research 2003 Jul;90(4):261-3
Parasitology research 2003 Jul;90(4):261-3
A new splice variant of glial fibrillary acidic protein, GFAP epsilon, interacts with the presenilin proteins.
Nielsen AL, Holm IE, Johansen M, Bonven B, Jørgensen P, Jørgensen AL
The Journal of biological chemistry 2002 Aug 16;277(33):29983-91
The Journal of biological chemistry 2002 Aug 16;277(33):29983-91
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of GFAP (arrow) using GFAP polyclonal antibody (Cat # PAB3851).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GFAP gene (Lane 2).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with GFAP polyclonal antibody (Cat # PAB3851) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
