14-9892-82

GFAP antibody from Invitrogen Antibodies
FLJ45472
Western blot
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Immunocytochemistry
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Immunohistochemistry
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Flow cytometry
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Antibody data

Product number
14-9892-82
Provider
Invitrogen Antibodies
Product name
Anti-GFAP Monoclonal Antibody (GA5), eBioscience™
Provider product page
Invitrogen Antibodies - 14-9892-82
Antibody type
Monoclonal
Antigen
Other
Description
Description: This GA5 monoclonal antibody reacts with human, mouse, rat, chicken, rabbit, and pig glial fibrillary acidic protein (GFAP). This 49-kDa type III intermediate filament protein is expressed in neural tissues and distinguishes astrocytes from other glial cells during central nervous system development. Three alternative splice variants of GFAP exist; however, alpha-GFAP is the predominant form expressed in astrocytes. GFAP can co-assemble with vimentin and nestin in astrocytes, but such associations are not required for assembly. Like other intermediate filaments, GFAP assembly is dependent on phosphorylation and dephosphorylation of the N-terminal domain. Studies have demonstrated that mutations in the GFAP gene lead to Alexander disease. Moreover, GFAP has also been shown to be overexpressed in certain glial-derived tumors. Applications Reported: This GA5 antibody has been reported for use in immunocytochemistry, western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and flow cytometric analysis. Applications Tested: This GA5 antibody has been tested by immunoblotting of lysate prepared from mouse brain, immunocytochemistry of fixed and permeabilized C6 cells, and immunohistochemistry of FFPE human tissue using low pH antigen retrieval. This can be used at less than or equal to 5 µg/mL. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human, Mouse, Rat, Chicken/Avian, Porcine, Rabbit
Host
Mouse
Isotype
IgG
Antibody clone number
GA5
Vial size
100 µg
Concentration
0.5 mg/mL
Storage
4° C
Provider Type Product Number
- No reagents -