Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- Ab105229 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- GFAP Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit anti Human GFAP Antibody, Polyclonal, could be used for WB, IHC and so on. Application WB: 0.01-3 µg/mL IHC: 5-30 µg/mL Protein Function GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 256-357- Vial size
- 100_l,10_l,1ml,20_l,50_l
- Concentration
- Lot by Lot
- Storage
- Store at 4_ short term (1-2 weeks). Store at -20_ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
No comments: Submit comment
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- GFAP Antibody (Ab105229) - Western Blot All lanes: GFAP Antibody (Ab105229) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution Predicted band size: 50 kDa Observed band size: 47, 53, 58 kDa Exposure time: 7.5 s
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- GFAP Antibody (Ab105229) - Western Blot All lanes: GFAP Antibody (Ab105229) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 50 kDa Observed band size: 44, 47,58 kDa Exposure time: 7.5 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- GFAP Antibody (Ab105229) - IHC DAB staining on IHC Samples: Human Cerebrum Tissue Primary Ab: 30 µg/mL GFAP Antibody (Ab105229) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
