AF2594

antibody from R&D Systems

Targeting: GFAP FLJ45472

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF2594

Provider

R&D Systems

Product name

Human/Rat GFAP Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human and rat GFAP in Western blots.

Reactivity

Human, Rat

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

P14136


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

APP upregulation contributes to retinal ganglion cell degeneration via JNK3.

Liu C, Zhang CW, Zhou Y, Wong WQ, Lee LC, Ong WY, Yoon SO, Hong W, Fu XY, Soong TW, Koo EH, Stanton LW, Lim KL, Xiao ZC, Dawe GS
Cell death and differentiation 2018 Mar;25(4):663-678

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Time-Dependent, HIV-Tat-Induced Perturbation of Human Neurons In Vitro: Towards a Model for the Molecular Pathology of HIV-Associated Neurocognitive Disorders.

Gurwitz KT, Burman RJ, Murugan BD, Garnett S, Ganief T, Soares NC, Raimondo JV, Blackburn JM
Frontiers in molecular neuroscience 2017;10:163

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Dose-dependent changes in neuroinflammatory and arachidonic acid cascade markers with synaptic marker loss in rat lipopolysaccharide infusion model of neuroinflammation.

Kellom M, Basselin M, Keleshian VL, Chen M, Rapoport SI, Rao JS
BMC neuroscience 2012 May 23;13:50

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TNFalpha-induced AMPA-receptor trafficking in CNS neurons; relevance to excitotoxicity?

Leonoudakis D, Braithwaite SP, Beattie MS, Beattie EC
Neuron glia biology 2004 Aug;1(3):263-73

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human and Rat GFAP by Western Blot. Western blot shows lysates of rat cortical stem cells, rat brain tissue, human brain (cortex) tissue, and human brain (hypothalamus) tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for GFAP at approximately 35-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human GFAP by Simple Western^TM. Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for GFAP at approximately 51 kDa (as indicated) using 0.1 µg/mL of Sheep Anti-Human/Rat GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Rat GFAP by Simple Western^TM. Simple Western lane view shows lysates of rat brain tissue, loaded at 0.2 mg/mL. A specific band was detected for GFAP at approximately 55 kDa (as indicated) using 2 µg/mL of Sheep Anti-Human/Rat GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

beta-III Tubulin in Rat Cortical Neurons and GFAP in Rat Astrocytes. beta-III Tubulin was detected in rat cortical neurons using 5 µg/mL Mouse Anti-neuron-specific Mouse beta-III Tubulin Monoclonal (clone TuJ-1) Antibody (Catalog # MAB1195). GFAP was detected in rat astrocytes using 10 µg/mL Sheep Anti-Human GFAP Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF2594). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for beta-III Tubulin using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and for GFAP using the Northern-Lights 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

GFAP in Rat Cortical Stem Cells. GFAP was detected in immersion fixed 7 days differentiated rat cortical stem cells using Sheep Anti-Human GFAP Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF2594) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (yellow; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

GFAP in Rat Astrocytes. GFAP was detected in immersion fixed rat astrocytes using 10 µg/mL Sheep Anti-Human GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.