HPA036760

antibody from Atlas Antibodies

Targeting: GCG GLP-1, GLP1, GLP2, GRPP

Antibody data

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Validation data

Product number: HPA036760 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA036760, RRID:AB_2675296
Product name: Anti-GCG
Antibody type: Polyclonal
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Antigen sequence: RSLQDTEEKSRSFSASQADPLSDPDQMNEDKRHSQ GTFTSDYSKYLDSRRAQDFVQWLMNTKRNRNNIAK RHDEF
Isotype: IgG
Vial size: 100 µl
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

GCG protein structure - HPA036760 shown in red.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
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Experimental details: Immunohistochemistry analysis in human pancreas and skeletal muscle tissues using HPA036760 antibody. Corresponding GCG RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Independent antibody validation
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Experimental details: Immunohistochemical staining of human colon, pancreas, skeletal muscle and small intestine using Anti-GCG antibody HPA036760 (A) shows similar protein distribution across tissues to independent antibody HPA036761 (B).
Antibody #2 product nr: HPA036761
Antibody provider: Atlas Antibodies

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in islet of Langerhans.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human pancreas shows high expression.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human liver shows low expression as expected.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human skeletal muscle shows no positivity in myocytes as expected.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in endocrine glandular cells.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in enteroendocrine cells.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in enteroendocrine cells.
Sample type: HUMAN