- PA5-13442 - Invitrogen Antibodies GCG antibody

Submitted references CD4(+) T cells regulate glucose homeostasis independent of adipose tissue dysfunction in mice.

Brinker G, Froeba J, Arndt L, Braune J, Hobusch C, Lindhorst A, Bechmann I, Gericke M
European journal of immunology 2021 Jun;51(6):1399-1411

The Protective Role of Prenatal Alpha Lipoic Acid Supplementation against Pancreatic Oxidative Damage in Offspring of Valproic Acid-Treated Rats: Histological and Molecular Study.

Ghoneim FM, Alrefai H, Elsamanoudy AZ, Abo El-Khair SM, Khalaf HA
Biology 2020 Aug 20;9(9)

Influence of Cannabinoid Receptor Deficiency on Parameters Involved in Blood Glucose Regulation in Mice.

Zibolka J, Wolf A, Rieger L, Rothgänger C, Jörns A, Lutz B, Zimmer A, Dehghani F, Bazwinsky-Wutschke I
International journal of molecular sciences 2020 Apr 30;21(9)

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis using a GCG polyclonal antibody (Product # PA5-13442) in mouse bladder tissue lysates (35 µg per lane).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue using a GCG polyclonal antibody (Product # PA5-13442), followed by HRP-conjugated secondary antibody and DAB staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of HepG2 cells using a GCG polyclonal antibody (Product # PA5-13442) (bottom), compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 Western blot analysis of insulin ( a ), glucagon ( b ) and somatostatin ( c ) protein in pancreatic tissue of male and female wild-type (Wt) mice and cannabinoid receptor knockout mouse lines (CB 1 -/- , CB 2 -/- ). ( a ) Insulin protein was decreased in the pancreatic tissue of CB 2 -/- mice. ( b ) Glucagon and ( c ) Somatostatin protein content displayed no significant differences, although a nonsignificant increase in CB 1 -/- was observed. Values are presented as mean (+-S.E.M.) with n = 8-16 mice per group. Each protein level of male and female Wt mice was defined as 100%. *** p < 0.001; unpaired t -test.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Photomicrographs of immunohistochemically stained pancreatic sections. Control sections ( a , b ). ( a ) An anti-insulin immune-reactivity of pancreatic islet showing brown-stained pancreatic beta cells with strong positive immune-reaction (arrowheads). ( b ) An anti-glucagon immune-reactivity of pancreatic islet showing brown-stained pancreatic alpha cells with mild positive immune-reaction (arrow). VPA treated pancreatic sections ( c , d ). ( c ) An anti-insulin immune-reactivity of pancreatic islet showing brown-stained pancreatic beta cells with mild positive immune-reaction (arrowheads). ( d ) An anti-glucagon immune-reactivity of pancreatic islet showing brown-stained pancreatic alpha cells with strong positive immune-reaction (arrow). VPA and ALA treated pancreatic sections ( e , f ). ( e ) An anti-insulin immune-reactivity of pancreatic islet showing brown-stained pancreatic beta cells with strong positive immune-reaction (arrowheads). ( f ) An anti-glucagon immune-reactivity of pancreatic islet showing brown-stained pancreatic alpha-cells with mild positive immune-reaction (arrow).

PA5-13442

Antibody data