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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- H00010687-M10 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PNMA2 Monoclonal Antibody (4F10)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Sequence of this protein is as follows: MALALLEDWC RIMSVDEQKS LMVTGIPADF EEAEIQEVLQ ETLKSLGRYR LLGKIFRKQE NANAVLLELL EDTDVSAIPS EVQGKGGVWK VIFKTPNQDT EFLERLNLFL EKEGQTVSGM FRALGQEGVS PATVPCISPE LLAHLLGQAM AHAPQPLLPM RYRKLRVFSG SAVPAPEEES FEVWLEQATE IVKEWPVTEA EKKRWLAESL RGPALDLMHI VQADNPSISV EECLEAFKQV FGSLESRRTA QVRYLKTYQE EGEKVSAYVL RLETLLRRAV EKRAIPRRIA DQVRLEQVMA GATLNQMLWC RLRELKDQGP PPSFLELMKV IREEEEEEAS FENESIEEPE ERDGYGRWNH EGDD
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4F10
- Vial size
- 100 µg
- Concentration
- See Label
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PNMA2 expression in transfected 293T cell line by PNMA2 monoclonal antibody (M10), clone 4F10.Lane 1: PNMA2 transfected lysate (Predicted MW: 41.5 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PNMA2 is approximately 3 ng/mL as a capture antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to PNMA2 on HeLa cell. Antibody concentration is 10 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of PNMA2 transfected lysate using anti-PNMA2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PNMA2 monoclonal antibody.
