HPA035565

antibody from Atlas Antibodies

Targeting: NEK5

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA035565

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA035565, RRID:AB_10672095

Product name

Anti-NEK5

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

ETLTFEDGMKFKEYECVKEHGDYTDKAFEKLHCPE
AGFSTQTVAAVGNRRQWDGGAPQTLLQMMAVADIT
STCPTGPDSESVLSVSRQEG


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human fallopian tube and prostate tissues using Anti-NEK5 antibody. Corresponding NEK5 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in spermatozoa and spermatids.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human fallopian tube shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human prostate shows low expression as expected.

Sample type

HUMAN