PA5-99456

antibody from Invitrogen Antibodies

Targeting: ACTA1 ACTA, NEM3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-99456

Provider

Invitrogen Antibodies

Product name

ACTA1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Antibody detects endogenous levels of total Actin alpha 1.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Skeletal Muscle Actin in mouse heart tissue lysate (left lane: treated with blocking peptide). Samples were incubated with Skeletal Muscle Actin polyclonal antibody (Product # PA5-99456).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Skeletal Muscle Actin in mouse heart tissue lysate (left lane: treated with blocking peptide). Samples were incubated with Skeletal Muscle Actin polyclonal antibody (Product # PA5-99456).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Skeletal Muscle Actin in various tissue sample (Lane 1: mouse heart tissue, Lane 2: mouse heart tissue, Lane 3: rat heart tissue, Lane 4: rat muscle). Samples were incubated with Skeletal Muscle Actin polyclonal antibody (Product # PA5-99456).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Skeletal Muscle Actin in HepG2 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Skeletal Muscle Actin polyclonal antibody (Product # PA5-99456) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of paraffin-embedded Skeletal Muscle Actin in human prostate tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Skeletal Muscle Actin polyclonal antibody (Product # PA5-99456) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.