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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB2578 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2578, RRID:AB_1570994
- Product name
- AHCY polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of AHCY.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Comparative kinetics of cofactor association and dissociation for the human and trypanosomal S-adenosylhomocysteine hydrolases. 2. The role of helix 18 stability.
Homocysteine-mediated expression of SAHH, DNMTs, MBD2, and DNA hypomethylation potential pathogenic mechanism in VSMCs.
Isozyme and DNA analysis of human S-adenosyl-L-homocysteine hydrolase (AHCY).
Li QS, Cai S, Fang J, Borchardt RT, Kuczera K, Middaugh CR, Schowen RL
Biochemistry 2008 Apr 29;47(17):4983-91
Biochemistry 2008 Apr 29;47(17):4983-91
Homocysteine-mediated expression of SAHH, DNMTs, MBD2, and DNA hypomethylation potential pathogenic mechanism in VSMCs.
Yideng J, Jianzhong Z, Ying H, Juan S, Jinge Z, Shenglan W, Xiaoqun H, Shuren W
DNA and cell biology 2007 Aug;26(8):603-11
DNA and cell biology 2007 Aug;26(8):603-11
Isozyme and DNA analysis of human S-adenosyl-L-homocysteine hydrolase (AHCY).
Arredondo-Vega FX, Charlton JA, Edwards YH, Hopkinson DA, Whitehouse DB
Annals of human genetics 1989 May;53(Pt 2):157-67
Annals of human genetics 1989 May;53(Pt 2):157-67
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of AHCY polyclonal antibody (Cat # PAB2578) in HepG2 cell line lysates (35 ug/lane). AHCY (arrow) was detected using the purified polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human colon carcinomareacted with AHCY polyclonal antibody (Cat # PAB2578), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
