10-4171
antibody from ABEOMICS Inc.
Targeting: CD68
DKFZp686M18236, GP110, LAMP4, macrosialin, SCARD1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- 10-4171 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-CD68 Antibody
- Antibody type
- Monoclonal
- Description
- CD68 is a transmembrane glycoprotein which acts as a commercialized marker for human monocytes and macrophages. Its expression is thought to be regulated by a macrophage-specific promoter. However, several immunohistochemical studies have indicated that CD68 antibodies also react with other haematopoietic and non-haematopoietic cell types. It also can stain myeloid cells, dendritic cells, fibroblasts, Langerhans cells and others. In human monocytes, which produce high levels of CD68 mRNA, the gene is characterized by intramolecular ligations between the promoter and the 3' intervening region. CD68 is also used as a pan-macrophage marker for TAM (Tumor-Associated Macrophages) which always involve in carcinogenesis.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
A partial length recombinant protei
n (a.a 80-280) of CD68 was used as
the immunogen for this antibody.- Isotype
- IgG
- Antibody clone number
- ABM53F5
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freez thawing
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Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of CD68. Anti-CD68 antibody (Clone : ABM53F5) was used at 2 µg/ml on human spleen lysate.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Cell surface Flow analysis of hCD68 in human PBMC (Monocytes) cells using 0.5 μg/ 10^6 cells. Green represents isotype control (ABEOMICS); red represents anti-hCD68 antibody (10-4171). Goat anti-mouse PE conjugated secondary antibody (ABEOMICS) was used.
- Protocol
- Protocol