PA1-29680

antibody from Invitrogen Antibodies

Targeting: LYZ

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-29680

Provider

Invitrogen Antibodies

Product name

Lysozyme Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Purifed from natural sources

Description

Predigestion with a proteolytic enzyme improves staining of formalin fixed paraffin embedded tissue sections.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

500 µL

Storage

4° C, do not freeze

References

Submitted references

A potent HNF4α agonist reveals that HNF4α controls genes important in inflammatory bowel disease and Paneth cells.

Lee SH, Veeriah V, Levine F
PloS one 2022;17(4):e0266066

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The Effect of Thiol Structure on Allyl Sulfide Photodegradable Hydrogels and their Application as a Degradable Scaffold for Organoid Passaging.

Yavitt FM, Brown TE, Hushka EA, Brown ME, Gjorevski N, Dempsey PJ, Lutolf MP, Anseth KS
Advanced materials (Deerfield Beach, Fla.) 2020 Jul;32(30):e1905366

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Relaxation of Extracellular Matrix Forces Directs Crypt Formation and Architecture in Intestinal Organoids.

Hushka EA, Yavitt FM, Brown TE, Dempsey PJ, Anseth KS
Advanced healthcare materials 2020 Apr;9(8):e1901214

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Impact of Maternal Malnutrition on Gut Barrier Defense: Implications for Pregnancy Health and Fetal Development.

Srugo SA, Bloise E, Nguyen TTN, Connor KL
Nutrients 2019 Jun 19;11(6)

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Ret receptor tyrosine kinase sustains proliferation and tissue maturation in intestinal epithelia.

Perea D, Guiu J, Hudry B, Konstantinidou C, Milona A, Hadjieconomou D, Carroll T, Hoyer N, Natarajan D, Kallijärvi J, Walker JA, Soba P, Thapar N, Burns AJ, Jensen KB, Miguel-Aliaga I
The EMBO journal 2017 Oct 16;36(20):3029-3045

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Kaiso differentially regulates components of the Notch signaling pathway in intestinal cells.

Robinson SC, Klobucar K, Pierre CC, Ansari A, Zhenilo S, Prokhortchouk E, Daniel JM
Cell communication and signaling : CCS 2017 Jun 21;15(1):24

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Paneth cell-mediated multiorgan dysfunction after acute kidney injury.

Park SW, Kim M, Kim JY, Ham A, Brown KM, Mori-Akiyama Y, Ouellette AJ, D'Agati VD, Lee HT
Journal of immunology (Baltimore, Md. : 1950) 2012 Dec 1;189(11):5421-33

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Lysozyme Rabbit Polyclonal Antibody (Product # PA1-29680) and a 15kDa band corresponding to Lysozyme was observed across cell lines tested except HEL 92.1.7, SK-BR-3 and SK-O-V3. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), THP-1 (Lane 2), HL-60 (Lane 3), HEL 92.1.7 (Lane 4), SK-BR-3 (Lane 5) and SK-O-V3 (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Lysozyme was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Lysozyme Rabbit Polyclonal Antibody (Product # PA1-29680) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing localization to golgi network and cytoplasm. Panel e shows SK-BR-3 cells with no expression of Lysozyme. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical (Paraffin) analysis of human tonsil tissue using (Product # PA1-29680) Lysozyme Polyclonal Antibody.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

10.1371/journal.pone.0266066.g004 Fig 4 NCT induced recovery of Paneth cells in intestine. A . Frozen sections of intestine from the mice described in Fig 1 were stained with HNF4alpha (green), Lysozyme (red) and DAPI (blue) in mice fed normal diet, HFD+DMSO or HFD+NCT. White box indicates high power view in adjacent panel. B. Quantification of the number of lysozyme-positive cells per intestinal crypt (HFD+DMSO vs. Normal or HFD+NCT). C, D . qPCR analysis in mouse small intestine of Plb1 and Defa22 mRNA expression normalized with 18s rRNA (N = 6-8). NS = non-significant, * p