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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [3]
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- Product number
- NB100-64803 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-64803, RRID:AB_964678
- Product name
- Mouse Monoclonal Myeloperoxidase/MPO Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. NB100-64803 recognizes human myeloperoxidase (MPO). MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55kD) and two light chains (15kD), the heavy chains being linked by a disulphide bond. NB100-64803 recognizes native MPO in Western blots, and the heavy chain following boiling of the sample. The antibody also recognizes recombinant MPO in Western blots and weakly in ELISA. The antibody may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry following cell permeablisation.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references IRF5 genetic risk variants drive myeloid-specific IRF5 hyperactivation and presymptomatic SLE.
Li D, Matta B, Song S, Nelson V, Diggins K, Simpfendorfer KR, Gregersen PK, Linsley P, Barnes BJ
JCI insight 2020 Jan 30;5(2)
JCI insight 2020 Jan 30;5(2)
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803] - Mouse anti Human Myeloperoxidase.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803] - Staining of human peripheral blood granulocytes with Mouse anti Human myeloperoxidase following permeabilisation with Leucoperm.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803] - An intracellular stain was performed on Jurkat cells with Myeloperoxidase antibody (2C7) NB100-64803PCP (blue) and a matched isotype control NBP2-27287PCP (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Peridinin-Chlorophyll-Protein complex.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803] - Analysis using the FITC conjugate of NB100-64803. Staining of permeabilised human peripheral blood granulocytes.
