100-4181

antibody from Rockland Immunochemicals, Inc.

Targeting: MPO

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

100-4181

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#100-4181, RRID:AB_2146468

Product name

Anti-MYELOPEROXIDASE (Human White Blood Cell) (RABBIT) Antibody - 100-4181

Antibody type

Polyclonal

Vial size

2 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Anti-Myeloperoxidase [Human Leukocytes] detects multiple MPO subunits and chain combinations by western blot.  Polyclonal rabbit-anti-Myeloperoxidase was used at a 1:5000 dilution to detect 1.0 ug of human myeloperoxidase. This antibody detects a multiple bands corresponding to 53 kDa and 15 kDa polypeptides and chain combinations forming 68 kDa and 106 kDa proteins.  The staining of the 68 kDa band is so intense that is over saturates the signal detection. A 4-20% gradient gel was used to separate the protein by SDS-PAGE.  The protein was transferred to nitrocellulose using standard methods.  After blocking the membrane was probed with the primary antibody for 2 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] (code 611-132-122) for 30 min at room temperature.  LICOR's Odyssey®  Infrared Imaging System was used to scan and process the image.  Other detection systems will yield similar results.

Validation comment

Western Blot