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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- MAB2772-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Primate Cripto-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and primate Cripto-1 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant mouse Cripto-1, recombinant human (rh) EGF, rhTGF-alpha , rhTGF-beta 1, rhTGF-beta 2, rhTGF-beta 3, or rhCryptic is observed.
- Reactivity
- Human, Simian
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P13385- Isotype
- IgG
- Antibody clone number
- 89633
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Cripto-1 in Human iPSCs. Cripto-1 was detected in immersion fixed human induced pluripotent stem cells (iPSCs) using Mouse Anti-Human/Primate Cripto-1 Monoclonal Antibody (Catalog # MAB2772) at 20 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
