Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF1538 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Cripto Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Cripto in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human Cripto-1 is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P51865- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cripto in D3 Mouse Cell Line. Cripto was detected in immersion fixed D3 mouse embryonic stem cell line using Sheep Anti-Mouse Cripto Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1538) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell secretion. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
