Explore
Validate
Learn600-401-997
antibody from Rockland Immunochemicals, Inc.
Targeting: TDGF1
CR, CR-1, CRIPTO, Cripto-1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 600-401-997 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-997, RRID:AB_2202665
- Product name
- Anti-Cripto-1 (TDGF1) (RABBIT) Antibody - 600-401-997
- Antibody type
- Polyclonal
- Vial size
- 100 µl
Submitted references An evaluation of different Cripto-1 antibodies and their variable results.
Cripto-1 vaccination elicits protective immunity against metastatic melanoma.
Translational significance of Nodal, Cripto-1 and Notch4 in adult nevi.
Age-Dependent Association between Protein Expression of the Embryonic Stem Cell Marker Cripto-1 and Survival of Glioblastoma Patients.
Cancer hallmarks in induced pluripotent cells: new insights.
Gudbergsson JM, Duroux M
Journal of cellular biochemistry 2020 Jan;121(1):545-556
Journal of cellular biochemistry 2020 Jan;121(1):545-556
Cripto-1 vaccination elicits protective immunity against metastatic melanoma.
Ligtenberg MA, Witt K, Galvez-Cancino F, Sette A, Lundqvist A, Lladser A, Kiessling R
Oncoimmunology 2016 May;5(5):e1128613
Oncoimmunology 2016 May;5(5):e1128613
Translational significance of Nodal, Cripto-1 and Notch4 in adult nevi.
Strizzi L, Margaryan NV, Gerami P, Haghighat Z, Harms PW, Madonna G, Botti G, Ascierto PA, Hendrix MJ
Oncology letters 2016 Aug;12(2):1349-1354
Oncology letters 2016 Aug;12(2):1349-1354
Age-Dependent Association between Protein Expression of the Embryonic Stem Cell Marker Cripto-1 and Survival of Glioblastoma Patients.
Tysnes BB, Satran HA, Mork SJ, Margaryan NV, Eide GE, Petersen K, Strizzi L, Hendrix MJ
Translational oncology 2013 Dec 1;6(6):732-41
Translational oncology 2013 Dec 1;6(6):732-41
Cancer hallmarks in induced pluripotent cells: new insights.
Malchenko S, Galat V, Seftor EA, Vanin EF, Costa FF, Seftor RE, Soares MB, Hendrix MJ
Journal of cellular physiology 2010 Nov;225(2):390-3
Journal of cellular physiology 2010 Nov;225(2):390-3
No comments: Submit comment
Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's affinity purified anti-Cripto-1 antibody shows detection of endogenous and transfected Cripto-1 from mouse and human sources. The Cripto-1 band appears above the 17.8 kDa marker. Endogenous detection is shown using mouse P19 embryonal carcinoma cells and human NCCIT testicular embryonal carcinoma cells. EpH4 CR-1 is a mouse mammary epithelial cell line stably expressing mouse Cripto-1. NTERA cells are human embryonal carcinoma cells that, when overgrown, differentiate and lose Cripto-1 expression. COS7 cells transfected with human Cripto-1 expression vector were used as a positive control and EpH4 WT cells were used as a negative control. A non-specific band at 45kDa may be present in some preparations. The primary antibody was used at a 1:500 dilution. Personal communication, C. Bianco, NCI, Bethesda, MD
- Validation comment
- Western Blot
