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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Flow cytometry [2]
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- Product number
- NB100-1598 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1598, RRID:AB_10000935
- Product name
- Rabbit Polyclonal CRIPTO Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references An evaluation of different Cripto-1 antibodies and their variable results.
MicroRNA-15b suppresses the growth and invasion of glioma cells through targeted inhibition of cripto-1 expression.
Lefty inhibits glioma growth by suppressing Nodal-activated Smad and ERK1/2 pathways.
Gudbergsson JM, Duroux M
Journal of cellular biochemistry 2020 Jan;121(1):545-556
Journal of cellular biochemistry 2020 Jan;121(1):545-556
MicroRNA-15b suppresses the growth and invasion of glioma cells through targeted inhibition of cripto-1 expression.
Sun G, Yan SS, Shi L, Wan ZQ, Jiang N, Fu LS, Li M, Guo J
Molecular medicine reports 2016 Jun;13(6):4897-903
Molecular medicine reports 2016 Jun;13(6):4897-903
Lefty inhibits glioma growth by suppressing Nodal-activated Smad and ERK1/2 pathways.
Sun G, Shi L, Li M, Jiang N, Fu L, Guo J
Journal of the neurological sciences 2014 Dec 15;347(1-2):137-42
Journal of the neurological sciences 2014 Dec 15;347(1-2):137-42
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CRIPTO Antibody [NB100-1598] - Total protein from human HeLa, NTERA-2 and spleen and embryonic mouse V6.5 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Cripto in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CRIPTO Antibody [NB100-1598] - An intracellular stain was performed on HeLa cells with NB100-1598PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CRIPTO Antibody [NB100-1598] - An intracellular stain was performed on Jurkat with NB100-1598 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.
