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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- PA1-16534 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cripto Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- In Western blot a band is seen at ~18 kDa representing the core form of Cripto1, and at ~36 kDa reporesenting the glycosylated/ post-translationally modified form.
- Concentration
- 1.0 mg/mL
Submitted references Activin A Modulates CRIPTO-1/HNF4α(+) Cells to Guide Cardiac Differentiation from Human Embryonic Stem Cells.
Duelen R, Gilbert G, Patel A, de Schaetzen N, De Waele L, Roderick L, Sipido KR, Verfaillie CM, Buyse GM, Thorrez L, Sampaolesi M
Stem cells international 2017;2017:4651238
Stem cells international 2017;2017:4651238
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot detection of Cripto in 20 µg of MDA-MB21 lysate using Product # PA1-16534. 10 sec. ECL exposure.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Cripto in human HeLa, NTERA-2 and spleen and embryonic mouse V6.5 cells. Samples were incubated in Cripto polyclonal antibody (Product # PA1-16534) using a dilution of 2 µg/mL followed by an anti-rabbit HRP secondary antibody. Separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. Detection: chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cripto staining in of Hela cells detected with a Dylight 488 labeled secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FACS staining of NTERA-2 cells using Product # PA1-16534 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 CRIPTO-1 and HNF4 alpha expression profiles in ActA treated human ESCs during early cardiac differentiation. HNF4 alpha + endodermal-like cells had a higher expression level of CRIPTO-1 in the control and 50 and 100 ng/mL ActA condition compared to the 10 and 25 ng/mL ActA treatment. Flow cytometry analyses of HNF4 alpha and CRIPTO-1 expression after induction of human ESC-CM differentiation with the indicated concentrations of ActA. Representative example of three independent experiments.
