PA5-118136

antibody from Invitrogen Antibodies

Targeting: P4HA2 C-P4Halpha(II)

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-118136

Provider

Invitrogen Antibodies

Product name

P4HA2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using P4HA2 Polyclonal Antibody (Product # PA5-118136) at 1:500 dilution. Lane A: HeLa Whole Cell Lysate, Lane B: H1299 Whole Cell Lysate, Lane C: 293T Whole Cell Lysate, Lane D: PC12 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 61 kDa. (We are unsure of the identity of these extra bands).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of P4HA2 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with P4HA2 Polyclonal Antibody (Product # PA5-118136, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human P4HA2 in human epididymis with P4HA2 Polyclonal Antibody (Product # PA5-118136, 1:2,000 dilution, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

P4HA2 Immunoprecipitation using: Lane A: 0.5 mg HeLa Whole Cell Lysate 4 µL with P4HA2 Polyclonal Antibody (Product # PA5-118136) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: P4HA2 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1,000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 61 kDa.