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Immunocytochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [2]
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- Product number
- DM1211 - Provider product page
- Provider
- OriGene
- Product name
- Pregnancy Specific Glycoprotein 1 (PSG1) mouse monoclonal antibody, clone BAP3, Purified
- Antibody type
- Monoclonal
- Description
- Pregnancy Specific Glycoprotein 1 (PSG1) mouse monoclonal antibody, clone BAP3, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PSG1
- Isotype
- IgG
- Antibody clone number
- BAP3
- Vial size
- 100 µg
- Concentration
- 1,0 mg/ml
Submitted references Carcinoembryonic antigen and CD44 variant isoforms cooperate to mediate colon carcinoma cell adhesion to E- and L-selectin in shear flow.
Thomas SN, Zhu F, Schnaar RL, Alves CS, Konstantopoulos K
The Journal of biological chemistry 2008 Jun 6;283(23):15647-55
The Journal of biological chemistry 2008 Jun 6;283(23):15647-55
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Fig2: Members of the CEA family were expressed on BOSC cells after transient transfection with expres-sion vectors containing either the cDNA of CEACAM1,3,5,7 or 8. Recognition of CEACAM3 and of arecombinant transmembrane-anchored PSG1 fusion protein was tested on stably transfected HeLa cells.Expression of the constructs was confirmed with monoclonal antibodies known to recognise the cor-responding proteins (CEACAM1, 3 and 5: D14HD11; CEACAM7: CAC2; CEACAM8: 80H3; PSG: Brified BAP3 was tested on all CEACAM transfectants. A positive signal was onlyobtained with PSG expressing cells (red curves).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Fig.1: FACS analysis of CHO cells using BAP3 . CHO cells were transiently transfected with an expression vector encoding a recombinant, transmembrane-anchored form of PSG1(red curve) or an irrelevant protein (control transfectant: blackcurve). Binding of BAP3 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only withPSG1 transfected cells.
- Validation comment
- FC
