Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Flow cytometry [2]
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- Product number
- ABIN108731 - Provider product page
- Provider
- antibodies-online
- Proper citation
- Antibodies-Online Cat#ABIN108731, RRID:AB_10953753
- Product name
- anti-Pregnancy Specific beta-1-Glycoprotein 5 (PSG5) antibody
- Antibody type
- Monoclonal
- Antigen
- immunisation with extracted protein of human PSG
- Description
- Protein G
- Reactivity
- Human
- Isotype
- IgG
- Antibody clone number
- BAP3
- Vial size
- 100 uL
- Concentration
- 1 mg/mL
- Storage
- short term: 2°C - 8°C, long term: -20°C
- Handling
- Avoid repeated freezing and thawing.
Submitted references Carcinoembryonic antigen and CD44 variant isoforms cooperate to mediate colon carcinoma cell adhesion to E- and L-selectin in shear flow.
Murine CD9 is the receptor for pregnancy-specific glycoprotein 17.
Pregnancy-specific glycoproteins function as immunomodulators by inducing secretion of IL-10, IL-6 and TGF-beta1 by human monocytes.
Redefined nomenclature for members of the carcinoembryonic antigen family.
The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues.
Highly specific monoclonal antibody demonstrates that pregnancy-specific glycoprotein (PSG) is limited to syncytiotrophoblast in human early and term placenta.
[Isolation and purification of specific beta1-G-globulin].
Thomas SN, Zhu F, Schnaar RL, Alves CS, Konstantopoulos K
The Journal of biological chemistry 2008 Jun 6;283(23):15647-55
The Journal of biological chemistry 2008 Jun 6;283(23):15647-55
Murine CD9 is the receptor for pregnancy-specific glycoprotein 17.
Waterhouse R, Ha C, Dveksler GS
The Journal of experimental medicine 2002 Jan 21;195(2):277-82
The Journal of experimental medicine 2002 Jan 21;195(2):277-82
Pregnancy-specific glycoproteins function as immunomodulators by inducing secretion of IL-10, IL-6 and TGF-beta1 by human monocytes.
Snyder SK, Wessner DH, Wessells JL, Waterhouse RM, Wahl LM, Zimmermann W, Dveksler GS
American journal of reproductive immunology (New York, N.Y. : 1989) 2001 Apr;45(4):205-16
American journal of reproductive immunology (New York, N.Y. : 1989) 2001 Apr;45(4):205-16
Redefined nomenclature for members of the carcinoembryonic antigen family.
Beauchemin N, Draber P, Dveksler G, Gold P, Gray-Owen S, Grunert F, Hammarström S, Holmes KV, Karlsson A, Kuroki M, Lin SH, Lucka L, Najjar SM, Neumaier M, Obrink B, Shively JE, Skubitz KM, Stanners CP, Thomas P, Thompson JA, Virji M, von Kleist S, Wagener C, Watt S, Zimmermann W
Experimental cell research 1999 Nov 1;252(2):243-9
Experimental cell research 1999 Nov 1;252(2):243-9
The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues.
Hammarström S
Seminars in cancer biology 1999 Apr;9(2):67-81
Seminars in cancer biology 1999 Apr;9(2):67-81
Highly specific monoclonal antibody demonstrates that pregnancy-specific glycoprotein (PSG) is limited to syncytiotrophoblast in human early and term placenta.
Zhou GQ, Baranov V, Zimmermann W, Grunert F, Erhard B, Mincheva-Nilsson L, Hammarström S, Thompson J
Placenta 1997 Sep;18(7):491-501
Placenta 1997 Sep;18(7):491-501
[Isolation and purification of specific beta1-G-globulin].
Sokolov AV, Kozliaeva GA, Mesniankina NV, Tatarinov IuS
Voprosy meditsinskoi khimii 1978 Mar-Apr;24(2):240-4
Voprosy meditsinskoi khimii 1978 Mar-Apr;24(2):240-4
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- FACS analysis of CHO cells using BAP3. CHO cells were transiently transfected with an expression vector encoding a recombinant, transmembrane-anchored form of PSG1 (red curve) or an irrelevant protein (control transfectant: black curve). Binding of BAP3 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with PSG1 transfected cells.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Members of the CEA family were expressed on BOSC cells after transient transfection with expression vectors containing either the cDNA of CEACAM1,3,5,7 or 8. Recognition of CEACAM3 and of a recombinant transmembrane-anchored PSG1 fusion protein was tested on stably transfected HeLa cells. Expression of the constructs was confirmed with monoclonal antibodies known to recognise the corresponding proteins (CEA-CAM1, 3 and 5: D14HD11; CEACAM7: CAC2; CEACAM8: 80H3; PSG: BAP3; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G purified BAP3 was tested on all CEACAM transfectants. A positive signal was only obtained with PSG expressing cells (red curves).