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- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Immunocytochemistry [1]
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- Product number
- AF1033 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Cathepsin X/Z/P Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse Cathepsin X/Z/P in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human Cathepsin X/Z/P is observed and less than 2% cross-reactivity with recombinant mouse Cathepsin B, C, D, H, and L is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9WUU7- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Inhibition of cathepsin X reduces the strength of microglial-mediated neuroinflammation.
Dysregulation of junctional adhesion molecule-A contributes to ethanol-induced barrier disruption in intestinal epithelial cell monolayers.
Mannose 6 dephosphorylation of lysosomal proteins mediated by acid phosphatases Acp2 and Acp5.
Mice deficient in LMAN1 exhibit FV and FVIII deficiencies and liver accumulation of α1-antitrypsin.
Macrophages and cathepsin proteases blunt chemotherapeutic response in breast cancer.
Intra- versus extracellular effects of microglia-derived cysteine proteases in a conditioned medium transfer model.
Pišlar A, Božić B, Zidar N, Kos J
Neuropharmacology 2017 Mar 1;114:88-100
Neuropharmacology 2017 Mar 1;114:88-100
Dysregulation of junctional adhesion molecule-A contributes to ethanol-induced barrier disruption in intestinal epithelial cell monolayers.
Chopyk DM, Kumar P, Raeman R, Liu Y, Smith T, Anania FA
Physiological reports 2017 Dec;5(23)
Physiological reports 2017 Dec;5(23)
Mannose 6 dephosphorylation of lysosomal proteins mediated by acid phosphatases Acp2 and Acp5.
Makrypidi G, Damme M, Müller-Loennies S, Trusch M, Schmidt B, Schlüter H, Heeren J, Lübke T, Saftig P, Braulke T
Molecular and cellular biology 2012 Feb;32(4):774-82
Molecular and cellular biology 2012 Feb;32(4):774-82
Mice deficient in LMAN1 exhibit FV and FVIII deficiencies and liver accumulation of α1-antitrypsin.
Zhang B, Zheng C, Zhu M, Tao J, Vasievich MP, Baines A, Kim J, Schekman R, Kaufman RJ, Ginsburg D
Blood 2011 Sep 22;118(12):3384-91
Blood 2011 Sep 22;118(12):3384-91
Macrophages and cathepsin proteases blunt chemotherapeutic response in breast cancer.
Shree T, Olson OC, Elie BT, Kester JC, Garfall AL, Simpson K, Bell-McGuinn KM, Zabor EC, Brogi E, Joyce JA
Genes & development 2011 Dec 1;25(23):2465-79
Genes & development 2011 Dec 1;25(23):2465-79
Intra- versus extracellular effects of microglia-derived cysteine proteases in a conditioned medium transfer model.
Wendt W, Schulten R, Stichel CC, Lübbert H
Journal of neurochemistry 2009 Sep;110(6):1931-41
Journal of neurochemistry 2009 Sep;110(6):1931-41
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cathepsin X/Z/P in Mouse Splenocytes. Cathepsin X/Z/P was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse Cathepsin X/Z/P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1033) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
