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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AF8354 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse/Rat DDT Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse and rat DDT in direct ELISAs and Western blots.
- Reactivity
- Mouse, Rat
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
O35215- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Mouse and Rat DDT by Western Blot. Western blot shows lysates of mouse liver tissue, mouse testis tissue, mouse kidney tissue, rat liver tissue, rat testis tissue, and rat kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat DDT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8354) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for DDT at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
