STJ11103513

antibody from St John's Laboratory

Targeting: NKX2-1 BCH, NKX2A, TITF1, TTF-1, TTF1

Immunocytochemistry

Antibody data

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Validation data

Product number: STJ11103513 - Provider product page
Provider: St John's Laboratory
Product name: Anti-TTF1 antibody (STJ11103513)
Antibody type: Monoclonal
Description: Rabbit monoclonal antibody anti-TTF1 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Antigen sequence: NA
Epitope: NA
Isotype: IgG
Antibody clone number: NA
Vial size: NA
Concentration: NA
Storage: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Handling: NA

NKX2-1 protein structure - STJ11103513 shown in red.

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunofluorescence analysis of rat thyroid using TTF1 rabbit monoclonal antibody (STJ11103513) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunofluorescence analysis of mouse thyroid using TTF1 rabbit monoclonal antibody (STJ11103513) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human thyroid cancer using TTF1 rabbit monoclonal antibody (STJ11103513) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human lung using TTF1 rabbit monoclonal antibody (STJ11103513) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded mouse lung using TTF1 rabbit monoclonal antibody (STJ11103513) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded rat lung using TTF1 rabbit monoclonal antibody (STJ11103513) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA