BML-SA452-0100

antibody from Enzo Life Sciences

Targeting: NKX2-1 BCH, NKX2A, TITF1, TTF-1, TTF1

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

BML-SA452-0100

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#BML-SA452-0100, RRID:AB_2050477

Product name

alpha-Tubulin (K(Ac)40), pAb

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Liquid. In PBS.

Vial size

100 µl

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Treatment of A549 cells with the histone deacetylase inhibitor trichostatin A (TSA, 0.3 µM, 7 hr.) induces acetylation of alpha-tubulin-Lys40. Western blot analysis was performed with A549 cell extracts (100,000 cells per lane) from either vehicle treated (Control) or TSAtreated (+TSA) cells. All lanes were probed with a 1:1000 dilution of the primary antibody, anti-alpha-tubulin (K(Ac)40) (Prod. No. BML-SA452). For lanes 2 and 5 the primary antibody was preincubated with the immunogen peptide (Ac-QMPSD-K(Ac)-TIGGG[CNH2] and for lanes 3 and 6 it was preincubated with non-acetylated peptide Ac-QMPSDKTIGGG[C-NH2] (peptides: 0.5 µg/µl, antibody: 1:100 dilution, 37¡C, 1hr.). Secondary antibody for all lanes was donkey anti-rabbit-AP (1:2000; Jackson Immunoresearch) and the nitrocellulose membrane was developed with BCIP/NBT.