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- Antibody Data
- Antigen structure
- References [17]
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- Western blot [3]
- Immunoprecipitation [1]
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- Product number
- NB100-236 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-236, RRID:AB_2107663
- Product name
- Rabbit Polyclonal Glucose 6 Phosphate Dehydrogenase Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Chronic Endurance Exercise Impairs Cardiac Structure and Function in Middle-Aged Mice with Impaired Nrf2 Signaling.
A biphasic effect of TNF-α in regulation of the Keap1/Nrf2 pathway in cardiomyocytes.
Impaired transcriptional activity of Nrf2 in age-related myocardial oxidative stress is reversible by moderate exercise training.
Phospho-ΔNp63α/SREBF1 protein interactions: bridging cell metabolism and cisplatin chemoresistance.
AMPK regulates NADPH homeostasis to promote tumour cell survival during energy stress.
Control of hepatic nuclear superoxide production by glucose 6-phosphate dehydrogenase and NADPH oxidase-4.
High glucose inhibits glucose-6-phosphate dehydrogenase, leading to increased oxidative stress and beta-cell apoptosis.
Glucose 6-phosphate dehydrogenase is regulated through c-Src-mediated tyrosine phosphorylation in endothelial cells.
Biochemical and mechanical dysfunction in a mouse model of desmin-related myopathy.
Aldosterone induces superoxide generation via Rac1 activation in endothelial cells.
N-terminal mutant huntingtin associates with mitochondria and impairs mitochondrial trafficking.
Human alpha B-crystallin mutation causes oxido-reductive stress and protein aggregation cardiomyopathy in mice.
Transformation of human mesenchymal stem cells increases their dependency on oxidative phosphorylation for energy production.
Glucose-6-phosphate dehydrogenase deficiency decreases vascular superoxide and atherosclerotic lesions in apolipoprotein E(-/-) mice.
Diabetes causes inhibition of glucose-6-phosphate dehydrogenase via activation of PKA, which contributes to oxidative stress in rat kidney cortex.
Glucose-6 phosphate dehydrogenase deficiency decreases the vascular response to angiotensin II.
Increased myocardial dysfunction after ischemia-reperfusion in mice lacking glucose-6-phosphate dehydrogenase.
Shanmugam G, Narasimhan M, Conley RL, Sairam T, Kumar A, Mason RP, Sankaran R, Hoidal JR, Rajasekaran NS
Frontiers in physiology 2017;8:268
Frontiers in physiology 2017;8:268
A biphasic effect of TNF-α in regulation of the Keap1/Nrf2 pathway in cardiomyocytes.
Shanmugam G, Narasimhan M, Sakthivel R, Kumar R R, Davidson C, Palaniappan S, Claycomb WW, Hoidal JR, Darley-Usmar VM, Rajasekaran NS
Redox biology 2016 Oct;9:77-89
Redox biology 2016 Oct;9:77-89
Impaired transcriptional activity of Nrf2 in age-related myocardial oxidative stress is reversible by moderate exercise training.
Gounder SS, Kannan S, Devadoss D, Miller CJ, Whitehead KJ, Odelberg SJ, Firpo MA, Paine R 3rd, Hoidal JR, Abel ED, Rajasekaran NS
PloS one 2012;7(9):e45697
PloS one 2012;7(9):e45697
Phospho-ΔNp63α/SREBF1 protein interactions: bridging cell metabolism and cisplatin chemoresistance.
Huang Y, Bell LN, Okamura J, Kim MS, Mohney RP, Guerrero-Preston R, Ratovitski EA
Cell cycle (Georgetown, Tex.) 2012 Oct 15;11(20):3810-27
Cell cycle (Georgetown, Tex.) 2012 Oct 15;11(20):3810-27
AMPK regulates NADPH homeostasis to promote tumour cell survival during energy stress.
Jeon SM, Chandel NS, Hay N
Nature 2012 May 9;485(7400):661-5
Nature 2012 May 9;485(7400):661-5
Control of hepatic nuclear superoxide production by glucose 6-phosphate dehydrogenase and NADPH oxidase-4.
Spencer NY, Yan Z, Boudreau RL, Zhang Y, Luo M, Li Q, Tian X, Shah AM, Davisson RL, Davidson B, Banfi B, Engelhardt JF
The Journal of biological chemistry 2011 Mar 18;286(11):8977-87
The Journal of biological chemistry 2011 Mar 18;286(11):8977-87
High glucose inhibits glucose-6-phosphate dehydrogenase, leading to increased oxidative stress and beta-cell apoptosis.
Zhang Z, Liew CW, Handy DE, Zhang Y, Leopold JA, Hu J, Guo L, Kulkarni RN, Loscalzo J, Stanton RC
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 May;24(5):1497-505
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 May;24(5):1497-505
Glucose 6-phosphate dehydrogenase is regulated through c-Src-mediated tyrosine phosphorylation in endothelial cells.
Pan S, World CJ, Kovacs CJ, Berk BC
Arteriosclerosis, thrombosis, and vascular biology 2009 Jun;29(6):895-901
Arteriosclerosis, thrombosis, and vascular biology 2009 Jun;29(6):895-901
Biochemical and mechanical dysfunction in a mouse model of desmin-related myopathy.
Maloyan A, Osinska H, Lammerding J, Lee RT, Cingolani OH, Kass DA, Lorenz JN, Robbins J
Circulation research 2009 Apr 24;104(8):1021-8
Circulation research 2009 Apr 24;104(8):1021-8
Aldosterone induces superoxide generation via Rac1 activation in endothelial cells.
Iwashima F, Yoshimoto T, Minami I, Sakurada M, Hirono Y, Hirata Y
Endocrinology 2008 Mar;149(3):1009-14
Endocrinology 2008 Mar;149(3):1009-14
N-terminal mutant huntingtin associates with mitochondria and impairs mitochondrial trafficking.
Orr AL, Li S, Wang CE, Li H, Wang J, Rong J, Xu X, Mastroberardino PG, Greenamyre JT, Li XJ
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Mar 12;28(11):2783-92
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Mar 12;28(11):2783-92
Human alpha B-crystallin mutation causes oxido-reductive stress and protein aggregation cardiomyopathy in mice.
Rajasekaran NS, Connell P, Christians ES, Yan LJ, Taylor RP, Orosz A, Zhang XQ, Stevenson TJ, Peshock RM, Leopold JA, Barry WH, Loscalzo J, Odelberg SJ, Benjamin IJ
Cell 2007 Aug 10;130(3):427-39
Cell 2007 Aug 10;130(3):427-39
Transformation of human mesenchymal stem cells increases their dependency on oxidative phosphorylation for energy production.
Funes JM, Quintero M, Henderson S, Martinez D, Qureshi U, Westwood C, Clements MO, Bourboulia D, Pedley RB, Moncada S, Boshoff C
Proceedings of the National Academy of Sciences of the United States of America 2007 Apr 10;104(15):6223-8
Proceedings of the National Academy of Sciences of the United States of America 2007 Apr 10;104(15):6223-8
Glucose-6-phosphate dehydrogenase deficiency decreases vascular superoxide and atherosclerotic lesions in apolipoprotein E(-/-) mice.
Matsui R, Xu S, Maitland KA, Mastroianni R, Leopold JA, Handy DE, Loscalzo J, Cohen RA
Arteriosclerosis, thrombosis, and vascular biology 2006 Apr;26(4):910-6
Arteriosclerosis, thrombosis, and vascular biology 2006 Apr;26(4):910-6
Diabetes causes inhibition of glucose-6-phosphate dehydrogenase via activation of PKA, which contributes to oxidative stress in rat kidney cortex.
Xu Y, Osborne BW, Stanton RC
American journal of physiology. Renal physiology 2005 Nov;289(5):F1040-7
American journal of physiology. Renal physiology 2005 Nov;289(5):F1040-7
Glucose-6 phosphate dehydrogenase deficiency decreases the vascular response to angiotensin II.
Matsui R, Xu S, Maitland KA, Hayes A, Leopold JA, Handy DE, Loscalzo J, Cohen RA
Circulation 2005 Jul 12;112(2):257-63
Circulation 2005 Jul 12;112(2):257-63
Increased myocardial dysfunction after ischemia-reperfusion in mice lacking glucose-6-phosphate dehydrogenase.
Jain M, Cui L, Brenner DA, Wang B, Handy DE, Leopold JA, Loscalzo J, Apstein CS, Liao R
Circulation 2004 Feb 24;109(7):898-903
Circulation 2004 Feb 24;109(7):898-903
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] - Detection of Human and Mouse G6PD by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (15 or 25 mcg for human; 20 mcg for mouse WB; 1 mg for mouse IP/WB) from human umbilical vein endothelial cells or mouse peritoneal macrophages from wildtype or G6PD deficient mice. Antibody: Affinity purified rabbit anti-G6PD used at 0.6 mcg/ml (A) and 0.5 mcg/ml (B) for WB or 3.75 mcg/mg lysate for IP (B). Detection: Chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] - Detection of Human and Mouse G6PD by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G6PD antibody NB100-236 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] - Immunoblot analyses of protein expression for Nrf2 and antioxidant enzymes. Representative immunoblots of cytosolic extracts from the hearts of young and old mice under basal conditions and following EES. Protein blots were probed with anti-HO1, NQO1, GCLM, GCLC, Catalase, SOD1, SOD2, GSR, G6PD, GPX1 and GAPDH. Individual lanes indicate a single animal. Densitometry analysis of respective protein signals was performed using Image-J and expressed as relative intensity units calculated as mean values of young and old, *p
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] - Detection of mouse G6PD by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-G6PD antibody NB100-236 (lot 2) used for IP at 6 ug per reaction. G6PD was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated G6PD, NB100-236 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
