NB600-302

antibody from Novus Biologicals

Targeting: MYC bHLHe39, c-Myc, MYCC

ELISA

Immunocytochemistry

Immunoprecipitation

Chromatin Immunoprecipitation

Antibody data

Submit

Validation data

Product number: NB600-302 - Provider product page
Provider: Novus Biologicals
Proper citation: Novus Cat#NB600-302, RRID:AB_2037060
Product name: Mouse Monoclonal c-Myc Antibody
Antibody type: Monoclonal
Description: Protein G purified. Specific for the c-myc protein in random coil configuration, not as a helix. 9E10 does not react with V-myc.
Reactivity: Human, Mouse, Drosophila
Host: Mouse
Antigen sequence: Leucine zipper sequence (AEEQKLISEE DLL)
Isotype: IgG
Vial size: 0.1 ml
Concentration: 1 mg/ml
Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

MYC protein structure - NB600-302 shown in red.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - Analysis of transgenic Eu-Myc+ mouse splenocytes using c-Myc PE conjugated antibody. Image from verified customer review.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - Analysis of Alexa Fluor (R) 647 conjugate of NB600-302. An intracellular stain was performed on HeLa cells with c-MYC antibody (9E10) NB600-302AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to AF647

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E10) NB600-302AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow (Intracellular): c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E10) NB600-302F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow (Intracellular): c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E10) NB600-302PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - Gray: WT B cell control Blue: EuMyc un-transformed B cells Red: EuMyc lymphoma This Image was submitted by customer review. Image from the FITC version of this antibody.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on HeLa cells with c-Myc Antibody (9E10) NB600-302 and a matched isotype control NBP1-43319. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on A431 cells with c-Myc Antibody (9E10) NB600-302AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - Gray: WT B cell control. Blue: EuMyc un-transformed B cells. Red: EuMyc lymphoma. This image was submitted via customer review. Image from the FITC version of this antibody.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on Jurkat cells with c-Myc Antibody (9E10) NB600-302R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on HeLa cells with c-Myc Antibody [9E10] NB600-302C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on K562 cells with c-Myc (9E10) antibody NB600-302PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.