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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Product number
- 18824-01 - Provider product page
- Provider
- QED Bioscience
- Product name
- Anti-c-myc Antibody (18824-01)
- Antibody type
- Polyclonal
- Description
- Anti-c-myc Goat Polyclonal Antibody
- Host
- Goat
- Epitope
- Amino acids 410-419 (EQKLISEEDL) of human myc conjugated to KLH.
- Vial size
- 100ug
- Concentration
- 1.0 mg/mL
- Storage
- This antibody is stable for 1 month at 4°C or one year at -20°C. Avoid multiple freeze-thaw cycles.
- Handling
- Dilute in PBS or medium that is identical to that used in the assay system.Immunoblotting: use at a dilution of 1:1,000-1:20,000. Detection of c-myc-tagged protein in 200, 100, and 50ng of E. coli lysate containing tagged fusion protein with #18824 diluted 1:25,000. Immunocytochemistry: use at a dilution of 1:100-1:400. ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100- 1:500. Immunoprecipitation: use at 1-4ug/mg lysate. These are recommended dilutions. Endusers should determine optimal dilutions for their applications.
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