Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB4284 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Claudin-12 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Claudin-12. Stains human Claudin-12 transfectants but not irrelevant transfectants.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NP_036261
- Isotype
- IgG
- Antibody clone number
- 393314
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Claudin-12 in NS0 Mouse Cell Line transfected with human Claudin-12 Claudin-12 was detected in immersion fixed NS0 mouse myeloma cell line transfected with human Claudin 12 using 10 µg/mL Mouse Anti-Human Claudin-12 Monoclonal Antibody (Catalog # MAB4284) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Claudin-12 in Caco-2 Human Cell Line Caco-2 human colorectal adenocarcinoma cell line was stained with Mouse Anti-Human Claudin-12 Monoclonal Antibody (Catalog # MAB4284, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).