Antibody data
- Antibody Data
- Antigen structure
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- Product number
- 42040 - Provider product page
- Provider
- QED Bioscience
- Product name
- Anti-Fibronectin Antibody (42040)
- Antibody type
- Monoclonal
- Description
- Anti-Human Fibronectin Mouse Monoclonal Antibody
- Reactivity
- Human, Bovine
- Host
- Mouse
- Epitope
- Human plasma fibronectin
- Isotype
- IgG
- Antibody clone number
- FN12-8
- Vial size
- 400ug
- Concentration
- Lot Specific
- Storage
- This antibody is stable for 1 month at 4°C or one year at -20°C. Avoid multiple freeze-thaw cycles.
- Handling
- Dissolve lyophilized antibody in 200ul of distilled water (final concentration 2.0mg/ml).ELISA: 1-10ug/ml with immobilized antigen. Western Blot: 1-10ug/ml, reducing or non-reducing conditions. Cell Adhesion Inhibition Assay: 0.1-1.0ug/ml on 10ug/ml fibronectin-coated microwells inhibits adhesion of trypsinized NRK cells. Immunohistochemistry: 1-10ug/ml, paraffin-embedded or frozen tissue sections with Proteinase K treatment for antigen retrieval. Staining of fibronectin (FN) in human well-differentiated colon cancer tissue (bottom) and moderately- differentiated colon cancer (top) with #42040. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.
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