Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations [0]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 42042 - Provider product page
- Provider
- QED Bioscience
- Product name
- Anti-Fibronectin Antibody (42042)
- Antibody type
- Monoclonal
- Description
- Anti-Human Fibronectin Mouse Monoclonal Antibody
- Reactivity
- Human
- Host
- Mouse
- Epitope
- Human plasma fibronectin
- Isotype
- IgG
- Antibody clone number
- FN30-8
- Vial size
- 400ug
- Concentration
- Lot Specific
- Storage
- The stock solution (2.0mg/ml) can be stored in aliquots at -20C for 1 year or can be stored at 4C for 6 months after adding 0.1% sodium azide. Prepare dilutions of stock solution immediately before use. Do not store diluted stock solution. Avoid repeated freeze-thaw cycles.
- Handling
- Dissolve lyophilized antibody in 200ul of distilled water (final concentration 2.0mg/ml).ELISA: 1-10ug/ml with immobilized antigen. Western Blot: 1-10ug/ml, reducing or non-reducing conditions. Cell Adhesion Inhibition Assay: 0.1-1.0ug/ml on 10ug/ml fibronectin-coated microwells inhibits adhesion of trypsinized NRK cells. Immunohistochemistry: 1-10ug/ml, paraffin-embedded or frozen tissue sections with Proteinase K treatment for antigen retrieval. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.
No comments: Submit comment
No validations: Submit validation data