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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- Ab134682 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant Wilms Tumor Protein Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human Wilms Tumor Protein Antibody, Recombinant (R05-2C6), could be used for WB, IHC and so on.ApplicationWB: 1/500-1/1000IHC: 1/50-/100Protein FunctionTranscription factor that plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 60-250- Antibody clone number
- R05-2C6
- Vial size
- 100μl,10μl,1ml,50μl
- Concentration
- 0,6 mg/ml
- Storage
- Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Wilms Tumor Protein Antibody (Ab134682) - Western Blot All lanes: Recombinant Wilms Tumor Protein Antibody (Ab134682) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 49 kDa Observed band size: 54 kDa Exposure time: 100.0 s
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Wilms Tumor Protein Antibody (Ab134682) - Western Blot All lanes: Recombinant Wilms Tumor Protein Antibody (Ab134682) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 49 kDa Observed band size: 52, 60 kDa Exposure time: 52.9 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Wilms Tumor Protein Antibody (Ab134682) - IHC Immunohistochemistry analysis of paraffin-embedded mouse kidney using Recombinant Wilms Tumor Protein Antibody (Ab134682). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
