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LearnNBP1-76628
antibody from Novus Biologicals
Targeting: C1QTNF3
2310005P21Rik, Corcs, Cors, Cors-26, CTRP3
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
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- Product number
- NBP1-76628 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-76628, RRID:AB_11001924
- Product name
- Rabbit Polyclonal C1qTNF3/CORS26/CTRP3 Antibody
- Antibody type
- Polyclonal
- Description
- Peptide affinity purified. Multiple isoforms of CTRP3 are known to exist. CTRP3 antibody is predicted to not recognize other members of the CTRP protein family.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: C1qTNF3/CORS26/CTRP3 Antibody [NBP1-76628] - Western Blot Validation in Mouse Heart Tissue Lysate in the (A) absence and (B) presence of blocking peptide. Loading: 15 ug of lysates per lane. Antibodies: C1qTNF3/CORS26/CTRP3, NBP1-76628 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: C1qTNF3/CORS26/CTRP3 Antibody [NBP1-76628] - Western blot analysis of CTRP3 in mouse heart cell lysate with C1qTNF3/CORS26/CTRP3 antibody at 1 ug/mL in the (A) absence and (B) presence of blocking peptide.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: C1qTNF3/CORS26/CTRP3 Antibody [NBP1-76628] - Western Blot Validation in Mouse and Rat Tissue Lysates. Loading: 15 ug of lysates per lane. Antibodies: C1qTNF3/CORS26/CTRP3, NBP1-76628 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: C1qTNF3/CORS26/CTRP3 Antibody [NBP1-76628] - Loading: 30 ng of human CTRP3 recombinant protein per lane. Antibodies: C1qTNF3/CORS26/CTRP3, NBP1-76628 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: C1qTNF3/CORS26/CTRP3 Antibody [NBP1-76628] - Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-C1qTNF3/CORS26/CTRP3 antibody (NBP1-76628) at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
